Journal: Cell reports

Article Title: BATF2 is a regulator of interferon-γ signaling in astrocytes during neuroinflammation

doi: 10.1016/j.celrep.2025.115393

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: IRF-1 (D5E4) XP ® Rabbit mAb , Cell Signaling , AB_10949108.

Techniques: Recombinant, Control, Protease Inhibitor, Western Blot, Electron Microscopy, Amplification, Reverse Transcription, SYBR Green Assay, Bicinchoninic Acid Protein Assay, RNA Sequencing, Software, Real-time Polymerase Chain Reaction

Journal: Virology

Article Title: Lipopolysaccharide restricts murine norovirus infection in macrophages mainly through NF-kB and JAK-STAT signaling pathway.

doi: 10.1016/j.virol.2020.04.010

Figure Lengend Snippet: Fig. 5. NF-ĸB signaling is important for LPS- induced IFN-β and ISG transcription. Cells were untreated or treated with LPS (100 ng/ ml), Bay 11–7085 (5 μM) or combination for 6 h qRT-PCR analysis of IFN-β (A) and ISG (B) mRNA levels in RAW264.7 cells (n = 6). qRT- PCR analysis of IFN-β (C) and ISG (D) mRNA levels in J774A.1 cells (n = 6). Western blot- ting analysis of STAT1 expression and phos- phorylation, and expression of IRF1 and GBP2 in RAW264.7 cells (E) and J774A.1 cells (F) untreated or treated with LPS (100 ng/ml), IFN-γ (100 U/ml), Bay 11–7085 (5 μM) or combination for 6 h qRT-PCR analysis of mRNA levels of IFN-β (G) (n = 6) and ISGs (H) (n = 6) in WT and NF-ĸB−/−MEFs untreated or treated with LPS (100 ng/ml) for 6 h. Data in (A, B, C, D and G) were normalized to the untreated control (CTR, set as 1). Data in (H) were normalized to untreated WT and NF- ĸB−/−MEFs, respectively (both set as 1). *P < 0.05; **P < 0.01. β-actin was used as a loading control.

Article Snippet: Antibodies against phosphoSTAT1 (Ser727) (#9177), STAT1 (#9172), MDA5 (D74E4, #5321) and IRF1 (D5E4, #8478), were purchased from Cell Signaling Technology.

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Control

Journal: Journal of Virology

Article Title: Differential Responses by Human Respiratory Epithelial Cell Lines to Respiratory Syncytial Virus Reflect Distinct Patterns of Infection Control

doi: 10.1128/JVI.02202-17

Figure Lengend Snippet: Summary of differential gene expression kinetics between A549 and BEAS-2B cells in response to low doses of RSV

Article Snippet: Levels of IRF1, IRF9, and RIG-I were visualized by chemiluminescence using rabbit anti-IRF1 monoclonal antibody (MAb) (clone D5E4; Cell Signaling Technology), rabbit anti-ISGF-3γ polyclonal antibody (PAb) (Santa Cruz, Dallas, TX), mouse anti-RIG-I MAb (clone 1c3; Millipore), and mouse anti-β-actin MAb (clone C4; Santa Cruz) as a control for protein loading.

Techniques: Expressing

Journal: Journal of Virology

Article Title: Differential Responses by Human Respiratory Epithelial Cell Lines to Respiratory Syncytial Virus Reflect Distinct Patterns of Infection Control

doi: 10.1128/JVI.02202-17

Figure Lengend Snippet: RSV-infected BEAS-2B cells express more IRF-1 but activate less IRF-3 and NF-κB than A549 cells. A549 or BEAS-2B cells were mock infected (medium only) or infected with rgRSV at an MOI of 0.1 or 0.3 for the indicated times. (A) Transcription factor gene expression was measured by RT-qPCR. The data shown are means ± SD from the results of four experiments performed in duplicate. Statistical differences between cell lines at each time point were calculated using a one-way ANOVA model without adjustment for mock infection on log10-transformed values as described in Materials and Methods. *, P < 0.05 for A549 compared to BEAS-2B mock infection at 0 h; ++, P < 0.05 for A549 compared to BEAS-2B at MOIs of 0.1 and 0.3; +, P < 0.05 at an MOI of either 0.1 or 0.3. (B and D) IRF3 dimerization (B) and IRF1 protein expression (D) were measured by Western blotting. The data represent the results of experiments on two separate cultures. (C) EMSA was performed to determine NF-κB activation.

Article Snippet: Levels of IRF1, IRF9, and RIG-I were visualized by chemiluminescence using rabbit anti-IRF1 monoclonal antibody (MAb) (clone D5E4; Cell Signaling Technology), rabbit anti-ISGF-3γ polyclonal antibody (PAb) (Santa Cruz, Dallas, TX), mouse anti-RIG-I MAb (clone 1c3; Millipore), and mouse anti-β-actin MAb (clone C4; Santa Cruz) as a control for protein loading.

Techniques: Infection, Expressing, Quantitative RT-PCR, Transformation Assay, Western Blot, Activation Assay